Today we finally began our experiment! I was super nervous, but I think we did pretty well with it. We had a lot of healthy cells in our wells at the end, and the picture to the right shows a magnified shot of our 6th plate, with 3.75 mLs of C2C12(undifferentiated mouse muscle stem cells) and 1.25 mLs of MyC-CaP(Mouse prostate cancer cells). The step we took today was only the first, but it was definitely the hardest. First, we took cells from inside the flasks, freed them from the bottom, and centrifuged those cells. After that, we disposed of the supernatant (the stuff on top) and re suspended the pellet(the stuff on the bottom) of cells into new media. We then put those in wells in our plate in varying amounts(look at our procedure for specifics). We were both very happy with our results, and we think that we did a good job. Our next step is to let them incubate for 24 hours, then document their growth. We're going to repeat that once(for solid data) and then dispose of them. We hope that the data we collect is going to benefit science, but my partner and I realize that it might be aiming too high. We're excited to see where our research takes us. Flowgram of our methods Our Calendar so far(look at end of October and Beginning of November) Updated Procedure
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AuthorAlex (Zachary)Wessel ArchivesCategories |